Monkey chants were aimed at three of Tottenham's black players, Jermain Defoe, Aaron Lennon and Andros Townsend during the game at White Hart Lane which ended in a 0-0 draw.
"UEFA has begun disciplinary proceedings against Lazio for 'improper conduct' by the club's supporters during Thursday's Europa League tie against Tottenham," said a UEFA statement.
"The Control and Disciplinary Body will meet on 18 October to discuss the case."
Tottenham boss Andre Villas-Boas had said after the match that UEFA should take action if there was a case to answer.
"UEFA is very active on this kind of situation so and our overall objective is to kick racism out of football," said Villas-Boas.
Source: AFP
